Glyoxalase I (GLX-I) analysis in native maize from Oaxaca, Mexico, infected with Aspergillus flavus in vitro

The glyoxalase system plays an important role in various physiological processes plants when they are subjected to different types of stress, whether physical, chemical or biological. Aspergillus flavus is aflatoxin-producing fungus that contaminates dry grains, leading a gradual deterioration the grains and significant reduction their nutritional value. objective present study was evaluate activity enzyme I (GLX-I) maize coleoptiles from Oaxaca response infection caused by flavus. Nine samples four races were analyzed. inoculated with suspension spores known concentration total protein extraction quantification performed on coleoptiles, GLX-I determined quantifying amount S-lactoylglutathione produced per minute. In addition, analysis gene expression reverse transcriptase polymerase chain reaction (RT-PCR) performed. showed symptoms infection, color changes wilting. proteins decreased significantly extracts presence fungus. analysis, two had highest enzymatic infected coleoptile extract respect healthy one, addition presenting greater RT-PCR assay, this due infection.